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Identification by Morphologic, Cultural, Biochemical, Serological, and Chromatographic Methods

Leon F. Ray, MD; Robert E. Kellum, MD

Arch Dermatol. 1970;101(1):36-40.

Abstract
Corynebacterium acnes can be identified if grown anaerobically on brain heart infusion agar with 1% polysorbate 80 (Tween 80) added. One millimeter dome-shaped, grayish-white colonies appear in three to five days. Smears reveal thin, gram-positive rods, singly or in pairs, and stain unevenly. A catalase test is positive. These colonies, subcultured on blood (sheep) agar, do not grow aerobically but flourish anaerobically as a profusion of pinpoint, translucent colonies at 37 C. Positive agglutinations are produced by C acnes antisera No. 554 or 605, or both. Gas chromatographic analysis of the bacterial fatty acids demonstrates a predominance of iso-C₁₅ branched fatty acid.

Author Affiliations
Portland, Ore

From the Division of Dermatology, University of Oregon Medical School, Portland, Ore. Dr. Kellum is currently at the Mason Clinic, Seattle.

Footnotes
Accepted for publication Aug 11, 1969.

Reprint requests to Division of Dermatology, University of Oregon Medical School, Portland, Ore 97201 (Dr. Ray).

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