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A Study Using the Fluorescent Periodic Acid—Acriflavine Method

Valter Winter, MD; Norman E. Levan, MD

Arch Dermatol. 1970;102(4):418-421.

Abstract
A stain combining periodic acid with the fluorochrome acriflavine (the fluorescent periodic acid-acriflavine [FPAA] reaction) was applied to specimens of normal adult human skin for study of the subepidermal basement membrane (BM). Advantages over conventional PAS technique were enhanced delineation of structural details and color contrast. In specimens from all body regions studied the BM showed variations in thickness, reticulation or stratification, and defects ranging from fragmentation to absence. These variations were least pronounced in plantar skin. Correlations of BM changes with basal layer morphology were noted.

Author Affiliations
Los Angeles

From the Section of Dermatology, University of Southern California, Los Angeles.

Footnotes
Accepted for publication March 10, 1970.

Reprint requests to 2025 Zonal Ave, Los Angeles 90033 (Dr. Levan).

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