This Article  • References  • Full text PDF  • Send to a friend  • Save in My Folder  • Save to citation manager  • Permissions  Citing Articles  • Citing articles on Web of Science (17)  • Contact me when this article is cited  Related Content  • Similar articles in this journal  Social Bookmarking   What's this?

Walter G. Klingler, MD; Paul M. Montague; Paul B. Chretien, MD; Vincent J. Hearing, PhD

Arch Dermatol. 1977;113(1):19-23.

Abstract
• Isolated melanosomes from human malignant melanoma lesions and normal tissues were solubilized with Triton X-100, and proteins were fractionated by polyacrylamide gel electrophoresis. Determination of the relative mobilities of protein bands at different acrylamide gel concentrations allowed the calculation of the molecular size and charge of melanosomal proteins. Three classes of proteins were demonstrable as follows: (a) those found only in melanoma; (b) those found only in normal melanosomes; and (c) proteins common to malignant and control tissues. The similarity of human melanoma protein banding patterns to those previously described in B-16 murine melanoma reaffirms the importance of the latter as a biochemical model for human studies; in addition, the presence of unique proteins in malignant tissue paralleled reports of aberrant polypeptides in organelles of several other tumor systems.

(Arch Dermatol 113:19-23, 1977)

Author Affiliations
From the Dermatology Branch (Drs Klingler, Montague, and Hearing) and Surgery Branch (Dr Chretien), National Cancer Institute, National Institutes of Health, Bethesda, MD.

Footnotes
Accepted for publication March 24, 1976.

Reprint requests to Bldg 10, Rm 12 N 254, National Institutes of Health, Bethesda, MD 20014 (Dr Hearing).

CiteULike    Connotea    Del.icio.us    Digg    Reddit    Technorati    Twitter     What's this?