This Article  • References  • Full text PDF  • Send to a friend  • Save in My Folder  • Save to citation manager  • Permissions  Citing Articles  • Citing articles on HighWire  • Contact me when this article is cited  Related Content  • Similar articles in this journal  Social Bookmarking   What's this?

Human Skin as a Substrate for Indirect Immunofluorescence Assay

David J. Goldberg, MD; Michael Sabolinski, MS; Jean-Claude Bystryn, MD

Arch Dermatol. 1985;121(9):1137-1140.

Abstract
• Human skin, the target organ for bullous pemphigoid (BP) antibodies, is thought to be a less sensitive substrate for the indirect immunofluorescence assay of BP antibodies than monkey or guinea pig esophagus. To examine the reasons for this puzzling phenomenon, we compared the titers of BP antibodies obtained when human skin, monkey, and guinea pig esophagus were used as substrates. We found the titers of BP antibodies obtained with human skin from sites commonly involved in BP (flexor arm, flexor thigh, popliteal fossa) were as high and usually higher than those obtained with monkey and guinea pig esophagus. In contrast, much lower titers were obtained with human skin from sites rarely involved in the disease (scalp, face, extensor arm). These findings suggest that human skin as a substrate is at least as sensitive as monkey or guinea pig esophagus for the indirect immunofluorescence assay of BP antibodies when the skin is obtained from regions on the body commonly involved in BP.

(Arch Dermatol 1985;121:1137-1140)

Author Affiliations
From the Department of Dermatology, New York University School of Medicine.

Footnotes
Accepted for publication Feb 22, 1985.

Reprint requests to Department of Dermatology, New York University School of Medicine, 560 First Ave, New York, NY 10016 (Dr Bystryn).

CiteULike    Connotea    Del.icio.us    Digg    Reddit    Technorati    Twitter     What's this?

THIS ARTICLE HAS BEEN CITED BY OTHER ARTICLES

Bullous Pemphigoid Antibodies
Accetta et al.
Arch Dermatol 1986;122:505-505.
ABSTRACT