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Gene Rearrangement Analysis of Tumor Cells in Tissue and Short-term Culture of a Patient

Higashi Sugimoto, MD; Fukiko Nakayama, MD; Takashi Yamauchi, MD; Yoshiki Tokura, MD; Keiji Iwatsuki, MD; Masahiro Takigawa, MD; Mizuho Yamada, MD; Michiyuki Maeda, MD

Arch Dermatol. 1988;124(3):405-408.

Abstract
• Cutaneous malignant lymphomas developed in an 80-year-old man without any evidence of leukemic disseminations of lymphoma cells. Immunohistologic staining showed the expression of Leu-3a, Leu-4, Ki-1, Tac, and HLA-DR antigens on tumor cells in tissue and large lymphoid cells in long-term, interleukin 2-containing culture of tumor explants. DNA samples extracted from the skin tumors and cultured lymphoid cells showed a clonal rearrangement of T-cell receptor (TCR) gene Cβ₁ with the molecular size of 9 kilobases. These findings suggested the T-cell origin of Ki-1⁺ cutaneous lymphoma cells and the occurrence of a clonal proliferation of tumor cells in culture.

(Arch Dermatol 1988;124:405-408)

Author Affiliations
From the Department of Dermatology, Hamamatsu (Japan) University School of Medicine (Drs Sugimoto, Nakayama, Iwatsuki, Takigawa, and Yamada); the Department of Dermatology, Hamamatsu (Japan) Red Cross Hospital (Dr Tokura); the Department of Dermatology, Shizuoka (Japan) General Hospital (Dr Yamauchi); and the Chest Disease Research Institute, Kyoto (Japan) University (Dr Maeda).

Footnotes
Accepted for publication Nov 4,1987.

Reprint requests to the Department of Dermatology, Hamamatsu University School of Medicine, 3600 Handa-cho, Hamamatsu 431-31, Japan (Dr Sugimoto).

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