This Article  • References  • Full text PDF  • Send to a friend  • Save in My Folder  • Save to citation manager  • Permissions  Citing Articles  • Citation map  • Citing articles on HighWire  • Citing articles on Web of Science (51)  • Contact me when this article is cited  Related Content  • Similar articles in this journal  Social Bookmarking   What's this?

A Multicenter Study

Michele De Luca, MD; Enrico Albanese, MD; Ranieri Cancedda, MD; Alberto Viacava, MD; Alessandro Faggioni, MD; Giovanna Zambruno, MD; Alberto Giannetti, MD

Arch Dermatol. 1992;128(5):633-638.

Abstract
• Background.—
In the past few years, several authors have described the usefulness of cultured allogeneic epidermal sheets in promoting wound healing of burns, leg ulcers, and donor sites. This study reports clinical results obtained by different departments in the treatment of chronic leg ulcers by cryopreserved cultured allogeneic epithelium. The freezing procedure and the assessment of viability of the cryopreserved epithelium are also described. A total of 30 ulcers were treated using 138 cryopreserved allografts.

Observations.—
Twenty ulcers (66.6%) healed completely within 12 weeks. Four ulcers showed a 30% to 84.4% reduction in size by 3 weeks but did not heal completely; the remaining six ulcers did not show any improvement. A strong stimulation of granulation tissue formation and of reepithelialization from the wound edge were observed.

Results.—
The results indicate that frozen cultured epidermis, stored in a skin bank, is a valid and generally applicable alternative therapy for the treatment of chronic ulcers.

(Arch Dermatol. 1992;128:633-638)

Author Affiliations
From the National Institute for Cancer Research, Laboratory of Cell Differentiation (Drs De Luca, Albanese, and Cancedda) and the General Hospital of Nervi (Dr Viacava and Faggioni), Genoa, Italy; and the Department of Dermatology, University of Modena (Italy) (Drs Zambruno and Giannetti).

Footnotes
Accepted for publication October 2, 1991.

Reprint requests to Laboratorio di Differenziamento Cellulare, IST, Istituto Nazionale per la Ricerca sul Cancro, Viale Benedetto XV no 10,16132, Genova, Italy (Dr De Luca).

CiteULike    Connotea    Del.icio.us    Digg    Reddit    Technorati    Twitter     What's this?

THIS ARTICLE HAS BEEN CITED BY OTHER ARTICLES

A Prospective Multicenter Study of the Efficacy and Tolerability of Cryopreserved Allogenic Human Keratinocytes to Treat Venous Leg Ulcers
Beele et al.
INT J LOW EXTREM WOUNDS 2005;4:225-233.
ABSTRACT  

Gene Therapy in the Treatment of Lower Extremity Wounds
Theopold et al.
INT J LOW EXTREM WOUNDS 2004;3:69-79.
ABSTRACT  

Cutaneous Tissue Engineering and Lower Extremity Wounds (Part 1)
Boschi et al.
INT J LOW EXTREM WOUNDS 2004;3:80-86.
ABSTRACT  

Risk Factors Associated With the Failure of a Venous Leg Ulcer to Heal
Margolis et al.
Arch Dermatol 1999;135:920-926.
ABSTRACT | FULL TEXT  

Rapid Healing of Venous Ulcers and Lack of Clinical Rejection With an Allogeneic Cultured Human Skin Equivalent
Falanga et al.
Arch Dermatol 1998;134:293-300.
ABSTRACT | FULL TEXT  

New Skin for Old: Developments in Biological Skin Substitutes
Phillips
Arch Dermatol 1998;134:344-349.
FULL TEXT  

Transforming Growth Factor Alpha Gene Expression in Cultured Human Keratinocytes Is Unaffected by Cellular Aging
Compton et al.
Arch Dermatol 1995;131:683-690.
ABSTRACT  

Chronic Skin Ulcers and the Biology of Skin Grafts
Harris
Arch Dermatol 1993;129:1204-1205.
ABSTRACT