This Article  • References  • Full text PDF  • Send to a friend  • Save in My Folder  • Save to citation manager  • Permissions  Citing Articles  • Citation map  • Citing articles on HighWire  • Contact me when this article is cited  Related Content  • Similar articles in this journal  Social Bookmarking   What's this?

Bruce R. Nelson, MD; Gopa Majmudar, PhD; Christopher E. M. Griffiths, MD; Montgomery O. Gillard; Anne E. Dixon, BM; Amir Tavakkol, PhD; Ted A. Hamilton, MS; Robert A. Woodbury, MD; John J. Voorhees, MD; Timothy M. Johnson, MD

Arch Dermatol. 1994;130(9):1136-1142.

Abstract
Background and Design
The ability of superficial dermabrasion to improve clinical features of photoaged skin is well known, but the specific biological mechanisms involved are poorly understood. The so-called repair zone, as visualized by routine histologic examination, has been attributed to new collagen formation within the papillary dermis and may be responsible for clinical improvement following dermabrasion. We investigated molecular and histologic events occurring in dermabraded skin and correlated them with clinical improvement. Ten photoaged patients (mean age, 59 years) underwent facial dermabrasion to the level of the papillary dermis. Clinical severity of photoaging was graded in a blinded manner at baseline and 12 weeks after dermabrasion. Biopsy specimens obtained at baseline and 3 and 12 weeks after dermabrasion were analyzed histologically and by in situ hybridization for fibroblast procollagen I mRNA, immunohistologically and by Western blotting with a monoclonal antibody specific for the aminoterminal cleavage site of procollagen I.

Results
Masson's trichrome staining demonstrated an increase in collagen from baseline (as an upper dermal band in the dermabrasion ''repair zone'') at 3 and 12 weeks' postdermabrasion. Immunohistologic examination demonstrated papillary dermal fibroblast staining for procollagen I at baseline that increased by threefold at 3 weeks' postdermabrasion and by 1.5-fold at 12 weeks' postdermabrasion. Western blotting demonstrated an average-fold increase in pN collagen I of 4.2± 1.5 at 3 weeks and of 2.7±0.7 at 12 weeks. By in situ hybridization, baseline levels of procollagen I mRNA in papillary dermal fibroblasts increased sixfold at weeks 3 and 12 postdermabrasion. Increase in procollagen I mRNA correlated with clinical improvement, ie, reduction in wrinkling.

Conclusion
Superficial dermabrasion clinically improves photoaged skin, and this improvement correlates strongly with increased collagen I gene expression.

(Arch Dermatol. 1994;130:1136-1142)

Author Affiliations
From the Departments of Dermatology (Drs Nelson, Majmudar, Griffiths, Dixon, Tavakkol, Woodbury, Voorhees, and Johnson and Messrs Gillard and Hamilton) and Otorhinolaryngology and Surgery (Plastic Surgery) (Drs Nelson and Johnson), University of Michigan, Ann Arbor. Dr Griffiths is now with the Department of Medicine, Section of Dermatology, University of Manchester, Salford, England, and Dr Tavakkol is with Hoffmann-LaRoche Inc, Nutley, NJ,

CiteULike    Connotea    Del.icio.us    Digg    Reddit    Technorati    Twitter     What's this?

THIS ARTICLE HAS BEEN CITED BY OTHER ARTICLES

Molecular Analysis of Aggressive Microdermabrasion in Photoaged Skin
Karimipour et al.
Arch Dermatol 2009;145:1114-1122.
ABSTRACT | FULL TEXT  

Treatment of Atrophic Facial Acne Scars With the 1064-nm Q-Switched Nd:YAG Laser: Six-Month Follow-up Study
Friedman et al.
Arch Dermatol 2004;140:1337-1341.
ABSTRACT | FULL TEXT  

Conventional Diamond Fraise vs Manual Spot Dermabrasion With Drywall Sanding Screen for Scars From Skin Cancer Surgery
Gillard et al.
Arch Dermatol 2002;138:1035-1039.
ABSTRACT | FULL TEXT  

Treatment of Perioral Rhytides: A Comparison of Dermabrasion and Superpulsed Carbon Dioxide Laser
Holmkvist and Rogers
Arch Dermatol 2000;136:725-731.
ABSTRACT | FULL TEXT  

Eyelid Healing After Carbon Dioxide Laser Skin Resurfacing: Histological Analysis
Mannor et al.
Arch Ophthalmol 1999;117:913-916.
ABSTRACT | FULL TEXT  

Combined Erbium:YAG and Carbon Dioxide Laser Skin Resurfacing
Millman and Mannor
Arch Facial Plast Surg 1999;1:112-116.
ABSTRACT | FULL TEXT  

Comparative Clinical Trial of 2 Carbon Dioxide Resurfacing Lasers With Varying Pulse Durations: 100 Microseconds vs 1 Millisecond
Duke et al.
Arch Dermatol 1998;134:1240-1246.
ABSTRACT | FULL TEXT  

Pilot Ultrastructural Evaluation of Human Preauricular Skin Before and After High-Energy Pulsed Carbon Dioxide Laser Treatment
Ratner et al.
Arch Dermatol 1998;134:582-587.
ABSTRACT | FULL TEXT  

Histologic Evaluation of Preauricular and Postauricular Human Skin After High-Energy, Short-Pulse Carbon Dioxide Laser
Cotton et al.
Arch Dermatol 1996;132:425-428.
ABSTRACT  

Therapeutic Dermabrasion: Back to the Future
Frank
Arch Dermatol 1994;130:1187-1189.
ABSTRACT