 |
|
Detection of Clonal T-Cell Receptor  Gene Rearrangements With the Use of the Polymerase Chain Reaction in Cutaneous Lesions of Mycosis Fungoides and Sézary Syndrome
Hervé Bachelez, MD;
Laurence Bioul;
Béatrice Flageul, MD;
Michel Baccard, MD;
Isabelle Moulonguet-Michau, MD;
Olivier Verola, MD;
Patrice Morel, MD;
Louis Dubertret, MD;
François Sigaux, MD
Arch Dermatol. 1995;131(9):1027-1031.
Abstract
| |
Background and Design
We used the amplification of junctional V (variable)-J (joining) sequences of the rearranged T-cell receptor  (TCR) genes by polymerase chain reaction for rapid and sensitive detection of a clonal T-cell population in a total of 51 skin specimens obtained from 45 patients with mycosis fungoides, five patients with Sézary syndrome, and 29 patients with chronic inflammatory dermatoses.
Results
A clonal TCR gene rearrangement was present in all tumors (3/3, 100%) and in most infiltrated plaques (16/22, 73%) and erythrodermas (10/12, 83%). In the patch stage, a clonal subset was found in more than half of the cases (8/14, 57%), whereas no clonality was observed in the controls. We also amplified the V-J sequences of the Igh locus coding for the heavy chain of immunoglobulins, without evidence of clonal rearrangement. These data were compared with those from in situ immunophenotypic analysis. Moreover, by using the same assay with successive dilutions of standard clonal T-cell DNA, a semiquantitative study of the T-cell clone was carried out in some cases. The highest ratios of clonal DNA were observed in advanced stages.
Conclusions
These data validate polymerase chain reaction V-J as a rapid, sensitive tool that can be used in the routine analysis of clonality in cutaneous lesions of mycosis fungoides and in the early diagnosis of mycosis fungoides and Sézary syndrome. Semiquantitative studies suggest that the malignant T-cell clone follows a selective process during the course of the progressive form of mycosis fungoides.
(Arch Dermatol. 1995;131:1027-1031)
Author Affiliations
From the Clinique des Maladies Cutanées (Drs Bachelez, Flageul, Moulonguet-Michau, and Dubertret), the Laboratoire d'Hématologie Moléculaire (Drs Bachelez and Sigaux and Ms Bioul), the Service de Dermatologie (Drs Baccard and Morel), and the Laboratoire d'Anatomie Pathologique (Dr Verola), Hôpital Saint-Louis, Paris, France.
 CiteULike  Connotea  Del.icio.us  Digg  Reddit  Technorati  Twitter
What's this?
THIS ARTICLE HAS BEEN CITED BY OTHER ARTICLES
|
Analysis of T-Cell Receptor Gene Rearrangement for Predicting Clinical Outcome in Patients With Cutaneous T-Cell Lymphoma: A Comparison of Southern Blot and Polymerase Chain Reaction Methods
Juarez et al.
Arch Dermatol 2005;141:1107-1113.
ABSTRACT
| FULL TEXT
Improved Sensitivity of T-Cell Clonality Detection in Mycosis Fungoides by Hand Microdissection and Heteroduplex Analysis
Dereure et al.
Arch Dermatol 2003;139:1571-1575.
ABSTRACT
| FULL TEXT
Diagnostic value of dominant T-cell clones in peripheral blood in 363 patients presenting consecutively with a clinical suspicion of cutaneous lymphoma
Delfau-Larue et al.
Blood 2000;96:2987-2992.
ABSTRACT
| FULL TEXT
The clinicopathological features of extensive small intestinal CD4 T cell infiltration
Carbonnel et al.
Gut 1999;45:662-667.
ABSTRACT
| FULL TEXT
A New Polymerase Chain Reaction-Based Method for the Detection of T-Cell Clonality in Patients With Possible Cutaneous T-Cell Lymphoma
Guitart and Kaul
Arch Dermatol 1999;135:158-162.
ABSTRACT
| FULL TEXT
The Clinical Use of Molecular Analysis of Clonality in Cutaneous Lymphocytic Infiltrates
Bachelez
Arch Dermatol 1999;135:200-202.
FULL TEXT
Are Complicated Forms of Celiac Disease Cryptic T-Cell Lymphomas?
Carbonnel et al.
Blood 1998;92:3879-3886.
ABSTRACT
| FULL TEXT
Prognostic Significance of a Polymerase Chain Reaction-Detectable Dominant T-Lymphocyte Clone in Cutaneous Lesions of Patients With Mycosis Fungoides
Delfau-Larue et al.
Blood 1998;92:3376-3380.
ABSTRACT
| FULL TEXT
Epstein-Barr Virus-Associated Lymphoproliferative Disease During Methotrexate Therapy for Psoriasis
Paul et al.
Arch Dermatol 1997;133:867-871.
ABSTRACT
Cutaneous T-Cell Lymphomas and Bacterial Superantigens
Musette et al.
Blood 1997;90:472-473.
FULL TEXT
T-Cell Gene Rearrangements in Mycosis Fungoides
Journal Watch Dermatology 1995;1995:7-7.
FULL TEXT
|
