Human Herpesvirus 8 DNA Sequences in Blistering Skin From Patients With Pemphigus

doi:10.1001/archderm.1997.03890460069008

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Vol. 133 No. 10, October 1997

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Human Herpesvirus 8 DNA Sequences in Blistering Skin From Patients With Pemphigus

Omeed M. Memar, MD, PhD; Peter L. Rady, MD, PhD; Randall M. Goldblum, MD; Angela Yen, MD; Stephen K. Tyring, MD, PhD

Arch Dermatol. 1997;133(10):1247-1251.

Abstract

Background
Human herpesvirus 8 (HHV-8) has been detected in Kaposi sarcoma(KS) and other lesions in patients both seropositive and seronegativefor the human immunodeficiency virus (HIV). Kaposi sarcoma hasbeen reported to develop in a disproportionate number of patientswith pemphigus. Since HHV-8 is so strongly associated with KS,we wondered whether HHV-8 is present in pemphigus lesions frompatients without KS or HIV infection. Pemphigus lesions andskin from healthy individuals were coded in a blinded fashion.Tissue-extracted DNA was tested using polymerase chain reaction,Southern blot hybridization, and automated sequencing of thepolymerase chain reaction products for the presence of HHV-8DNA. Six patients had pemphigus foliaceus, 6 had pemphigus vulgaris,and 2 had KS; 10 healthy individuals were used as controls.All 24 patients were HIV seronegative.

Observation
Lesional skin from 4 of the 6 patients with pemphigus vulgaris,all 6 of the patients with pemphigus foliaceus, and both positivecontrols (KS) tested positive for HHV-8 DNA. Furthermore, theHHV-8 DNA sequences for KS330₂₃₃ differed between all 6 DNAspecimens from pemphigus foliaceus, while 3 of the 4 DNA specimensfrom pemphigus vulgaris were identical. However, HHV-8 DNA wasabsent in all normal human skin analyzed.

Conclusions
This report expands the spectrum of lesions found to containHHV-8 DNA sequences and suggests that HHV-8 might have trophismfor pemphigus lesions.

Arch Dermatol. 1997;133:1247-1251

Author Affiliations

From the Departments of Microbiology and Immunology (Drs Memar, Rady, and Tyring), Pediatrics (Dr Goldblum), and Dermatology (Drs Yen and Tyring), The University of Texas Medical Branch, Galveston.

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