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Persistence of T-Cell Clones in Psoriatic Lesions
Jennie C. C. Chang, PhD;
Lawrence R. Smith, PhD;
Karen J. Froning;
Helen H. Kurland, MD;
Beverly J. Schwabe;
Kirsten K. Blumeyer;
Marvin A. Karasek, PhD;
David I. Wilkinson, PhD;
Eugene M. Farber, MD;
Dennis J. Carlo, PhD;
Steven W. Brostoff, PhD
Arch Dermatol. 1997;133(6):703-708.
Abstract
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Background
We previously demonstrated a clonal dominance in the Vβ13.1 messages isolated from the lesional CD8+ T cells of psoriasis vulgaris, which suggested an interaction of Vβ13.1+ CD8+ T cells with skin antigens.
Objectives
To determine whether the clonality observed accurately reflected a clonal population of infiltrating T cells or was skewed by an overabundance of messages from a small number of cells, and to extend our study of Vβ gene usage by lesional CD8+ T cells to 9 new patients.
Design
Case study.
Setting
Patients were enrolled at the Psoriasis Research Institute in Palo Alto, Calif, and samples were analyzed at The Immune Response Corporation in Carlsbad, Calif.
Main Outcome Measures
For the 2 previous patients, skin samples were sorted directly for Vβ13.1+ T cells, for which the T-cell receptors were sequenced. For the 9 new patients, CD8+ T cells were sorted and their T-cell receptor Vβ gene usage measured using semiquantitative polymerase chain reaction with Vβ-specific primers.
Results
The directly sorted Vβ13.1+ T cells exhibited clonal dominance in both patients. The dominant Vβ13.1 clone in each patient was the same as that found in the previous 2 biopsy specimens for which CD8+ T cells were sorted. Additionally, in 8 of the 9 new patients examined, we again found a preferential usage of Vβ3 and/or Vβ13.1 genes by the lesional CD8+ T cells.
Conclusions
The clonality, which was found in the Vβ messages of the sorted CD8+ T cells, accurately reflects the dominance of these clones in the infiltrating T cells. Moreover, the persistence in the same patient of the same clone for as long as 15 months and the overrepresentation of Vβ3 and/or Vβ13.1 in lesional CD8+ T cells in the new patients examined support the pathogenic role of T cells bearing these Vβs.
Arch Dermatol. 1997;133:703-708
Author Affiliations
From The Immune Response Corporation, Carlsbad, Calif (Drs Chang, Smith, Kurland, Carlo, and Brostoff and Mss Froning, Schwabe, and Blumeyer), and the Psoriasis Research Institute, Palo Alto, Calif (Drs Karasek, Wilkinson, and Farber).
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