 |
|
The Longevity of a Bilayered Skin Substitute After Application to Venous Ulcers
Tania J. Phillips, MD;
Jasmin Manzoor, MD;
Adriana Rojas, MD;
Cary Isaacs, MS;
Polly Carson, CWS;
Michael Sabolinski, MD;
Jan Young, PhD;
Vincent Falanga, MD
Arch Dermatol. 2002;138:1079-1081.
Background A bilayered skin substitute composed of allogeneic keratinocytes and
fibroblasts in a collagen gel has been approved by the US Food and Drug Administration
for the treatment of venous and diabetic ulcers. Its mechanism of action has
not been fully determined.
Objective To determine the longevity of allogeneic fibroblasts and keratinocytes
in a bilayered skin substitute in patients with venous leg ulcers.
Methods Ten patients with venous leg ulcers were treated with a bilayered skin
substitute on day 0, days 3 to 5, and weeks 1 through 3. Biopsy specimens
of the grafted wound were taken. We used polymerase chain reaction analysis
to determine whether allogeneic DNA was present in the biopsy specimens.
Results We detected allogeneic DNA in 2 of 8 specimens at 1 month after initial
grafting. Neither of the 2 patients showed persistence of allogeneic DNA at
2 months after initial grafting.
Conclusions Allogeneic cells from a bilayered skin substitute do not appear to survive
permanently after grafting for treatment of venous leg ulcers. Other mechanisms
of action might include cytokine release, structural support, or provision
of a moist wound environment.
From the Department of Dermatology, Boston University School of Medicine,
Boston, Mass (Drs Phillips, Manzoor, Rojas, and Falanga and Ms Carson), and
Organogenesis, Inc, Canton, Mass (Mr Isaacs and Drs Sabolinski and Young).
THIS ARTICLE HAS BEEN CITED BY OTHER ARTICLES
Vascularization and engraftment of a human skin substitute using circulating progenitor cell-derived endothelial cells
Shepherd et al.
FASEB J. 2006;20:1739-1741.
ABSTRACT
| FULL TEXT
Engraftment of a vascularized human skin equivalent
SCHECHNER et al.
FASEB J. 2003;17:2250-2256.
ABSTRACT
| FULL TEXT
Evidence for Living Cells: DNA Fragments Are Not Enough
Epstein
Arch Dermatol 2003;139:541-541.
FULL TEXT
Longevity of Bilayered Skin Substitute on Venous Leg Ulcers
Journal Watch Dermatology 2002;2002:2-2.
FULL TEXT
Tissue engineering and the trauma surgeon
Garner
Trauma 2002;4:177-183.
ABSTRACT
|
