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Difference Between Sun-Protected and Photodamaged Human Skin In Vivo

Laure Rittié, PhD; Sewon Kang, MD; John J. Voorhees, MD; Gary J. Fisher, PhD

Arch Dermatol. 2008;144(9):1129-1140.

Objective  To evaluate the effectiveness of topical estradiol in stimulating collagen I and III production in naturally aged and photoaged human skin of postmenopausal women and age-matched men.

Design  Vehicle-controlled treatment followed by biochemical and immunohistochemical analyses of skin biopsy specimens.

Setting  Academic referral center.

Participants  Seventy healthy volunteers (40 postmenopausal women with a mean age of 75 years, and 30 men with a mean age of 75 years) with photodamaged skin.

Interventions  Topical application of estradiol, 0.01%, 0.1%, 1%, or 2.5% or vehicle on aged or photoaged skin, with biopsy specimens taken after last treatment.

Main Outcome Measures  De novo synthesis of collagen by quantitative polymerase chain reaction, immunohistochemistry, and enzyme-linked immunosorbent assay.

Results  Topical estradiol increased procollagen I and III messenger RNA and collagen I protein levels in sun-protected aged hip skin in postmenopausal women and, to a lesser extent, in age-matched men. Surprisingly, no significant changes in production were observed in women or men after 2-week estradiol treatment of photoaged forearm or face skin, despite similar expression of estrogen receptors (ER-, ER-β, and GPR30) in aged and photoaged skin. Estradiol treatment induced the estrogen-responsive gene GREB1, indicating that penetration of topical estradiol and genomic response to estrogen were similar in the 3 anatomic sites.

Conclusions  Two-week topical estradiol treatment stimulates collagen production in sun-protected hip skin, but not in photoaged forearm or face skin, in postmenopausal women and aged-matched men. These findings suggest that menopause-associated estrogen decline is involved in reduced collagen production in sun-protected skin. Interestingly, alterations induced by long-term sun exposure hinder the ability of topical 2-week estradiol to stimulate collagen production in aged skin.

Trial Registration  clinicaltrials.gov Identifier: NCT00113100

Author Affiliations: Department of Dermatology, University of Michigan Medical School, Ann Arbor.

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