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ALBERT M. KLIGMAN, MD; ENNO CHRISTOPHERS, MD

Arch Dermatol. 1963;88(6):702-705.

Abstract
Summary

Methods of preparing intact, undenatured sheets of human stratum corneum are described. The epidermis of split or full-thickness skin is first separated by exposure to ammonia fumes or heat at 60 C. The horny layer is then isolated by incubating in dilute trypsin solution. Cantharidin blisters provide a convenient way of obtaining stratum corneum from living subjects with minimum discomfort and no scarring.

Author Affiliations
PHILADELPHIA

From the Department of Dermatology, University of Pennsylvania School of Medicine.

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