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Confirmatory Observations and Evaluation of Technical Considerations

DONALD S. WALDORF, MD; CHARLES W. SMITH, BS; ARTHUR J. L. STRAUSS, MD

Arch Dermatol. 1966;93(1):28-33.

Abstract
This study confirms the findings of Beutner and Jordan that patients with pemphigus vulgaris have circulating serum antibodies demonstrable by indirect immunofluorescent staining to the intercellular areas of certain epithelial tissues, including skins.

Several technical considerations in immunofluorescent techniques may favor or diminish the likelihood of obtaining reproducible results, especially when skin is used as a substrate. These problems are discussed in some detail.

Author Affiliations
BETHESDA, MD

From the Dermatology Branch, National Cancer Institute, Public Health Service, US Department of Health, Education, and Welfare, and the Laboratory of Immunology, National Institute of Allergy and Infectious Diseases.

Footnotes
Accepted for publication July 29, 1965.

Reprint requests to Dermatology Branch, National Cancer Institute, Public Health Service, Bethesda, Md 20014 (Dr. Waldorf).

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