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The Clinical Use of Molecular Analysis of Clonality in Cutaneous Lymphocytic Infiltrates
Arch Dermatol. 1999;135:200-202.
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WITHIN RECENT years, the emergence of molecular genetic techniques has provided new tools allowing the accurate analysis of the clonality status in both T-cell and B-cell infiltrates. The methods used for clonality assessment are based on the genomic organization of the genes coding for T-cell receptor (TCR) and immunoglobulin chains, which display a similar pattern characterized by clusters of exons separated by large intronic sequences, being rearranged at the DNA level during the maturation process of lymphocytes.1 Thus, the Southern blot analysis of genes coding for TCRB or for TCRG chains was the method used in initial studies that showed evidence for the monoclonal nature of cutaneous T-cell neoplasms, such as Sézary syndrome and mycosis fungoides (MF).2 However, Southern blot is a time-consuming method that requires the usage of radioactive oligonucleotidic probes and detects a monoclonal TCR rearrangement only if its ratio exceeds 5% of all TCR rearrangements among a . . . [Full Text of this Article]THE NATURAL HISTORY OF CUTANEOUS LYMPHOCYTIC INFILTRATES: LESSONS FROM CLONALITY STUDIES
HOW USEFUL ARE MOLECULAR CLONALITY ASSAYS IN THE DIAGNOSIS OF CUTANEOUS LYMPHOID INFILTRATES?
WHAT IS THE PROGNOSTIC SIGNIFICANCE OF THE DETECTION OF A MONOCLONAL SUBSET IN CUTANEOUS LYMPHOCYTIC INFILTRATES SHOWING BENIGN OR UNSPECIFIC HISTOPATHOLOGICAL FEATURES?
THE FUTURE OF CLONALITY ASSAYS
RELATED ARTICLE
A New Polymerase Chain Reaction–Based Method for the Detection of T-Cell Clonality in Patients With Possible Cutaneous T-Cell Lymphoma
Joan Guitart and Karen Kaul
Arch Dermatol. 1999;135(2):158-162.
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