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Amplification of DNA of Borrelia burgdorferi in Urine Samples of Patients With Granuloma Annulare and Lichen Sclerosus et Atrophicus
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The detection of Borrelia burgdorferi DNA by polymerase chain reaction (PCR) analysis in urine samples has gained importance in the laboratory diagnosis of Lyme borreliosis. In the recent years, dermatoses, such as certain forms of morphea, Shulman syndrome, and, more recently, granuloma annulare (GA) and lichen sclerosus et atrophicus (LSA), have been linked with B burgdorferi infection.
In our study, a nested PCR method was used to investigate the urine samples of 13 patients with GA and 19 patients with LSA. Two different gene segments of B burgdorferi DNA were amplified, one derived from a specific part of the flagellin and the other from the chromosomal RNA polymerase C gene.
Polymerase chain reaction analysis revealed flagellin gene sequences in 8 (61%) of the 13 patients with GA and 13 (68%) of the 19 patients with LSA; among these patients, both genes were present in 3 patients (38%) with GA and . . . [Full Text of this Article] Subjects, Materials, and Methods
Results
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