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Defining Wound Microbial Flora: Molecular Microbiology Opening New Horizons
Yelena M. Frankel, MD, MPH;
Johan H. Melendez, MS;
Nae-Yuh Wang, PhD;
Lance B. Price, PhD;
Jonathan M. Zenilman, MD;
Gerald S. Lazarus, MD
Arch Dermatol. 2009;145(10):1193-1195.
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Chronic wounds cause substantial morbidity.1 Although nonhealing is often attributed to infection, the process for ascertaining bacterial flora within wounds is not standardized. Qualitative swab cultures are characterized as showing few, moderate, or heavy bacterial presence, but the significance of these categories has not been validated.2 A culture is an insensitive test for detecting fastidious organisms. Therefore, to appreciate the role of bacteria in nonhealing wounds, careful characterization and clinical correlation is required.
We evaluated the bacterial ecology of chronic wounds in a prospective series of patients. We developed and standardized a convenient tissue sampling method, compared qualitative and quantitative culture results, and assessed the utility of real-time polymerase chain reaction (RT-PCR) for detecting methicillin-susceptible Staphylococcus aureus (MSSA) and methicillin-resistant S aureus (MRSA).
Methods
This study was . . . [Full Text of this Article]
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