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Arch Dermatol. 1991;127(8):1126-1127.

References
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1. CDC. Possible transmission of human immunodeficiency virus to a patient during an invasive dental procedure. MMWR 1990;39:489-93. PUBMED 2. CDC. Update: transmission of HIV infection during an invasive dental procedure—Florida. MMWR 1991;40:21-7, 33. PUBMED 3. Ou CY, Kwok S, Mitchell SW, et al. DNA amplification for direct detection of HIV-1 in DNA of peripheral mononuclear cells. Science 1988;239:295-7. FREE FULL TEXT 4. Grob P, Bischof B, Naeff R. Cluster of hepatitis B transmitted by a physician. Lancet 1981; 2:1218-20. PUBMED 5. Rimland D, Parkin WE, Miller GB, et al. Hepatitis B outbreak traced to an oral surgeon. N Engl J Med 1977;296:953-8. ABSTRACT 6. Ahtone J, Goodman RA. Hepatitis B and dental personnel: transmission to patients and prevention issues. J Am Dent Assoc 1983;106:219-22. ABSTRACT * In the initial sequencing of the HIV proviral DNA from patients E, F, and G, proviral DNA that had been amplified by the polymerase chain reaction (PCR) was molecularly cloned before it was sequenced. Unique sequences were included in the PCR primers used for amplification to distinguish the amplified product of each patient's specimen. To verify these results, additional blood samples obtained from patients F and G and a second aliquot of the initial blood sample from patient E were reanalyzed. In this reanalysis, amplified HIV DNA was sequenced directly, without molecular cloning. In each case, consensus sequences from the reanalysis were virtually identical to the initial sequence results. Sequencing of amplified proviral DNA from 24 control patients was also done directly. None of the proviral sequences from the dentist, patients A-G, and the 31 local controls were identical, indicating that the specimens had not been cross-contaminated. In addition, the proviral sequences from the dentist and the seven patients were reproduced in repeat analyses, providing further evidence of absence of crosscontamination.

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