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Its Application to Skin Biopsies

HERBERT H. JOHNSON, Jr., M.D.

AMA Arch Derm. 1955;72(4):307-312.

	Since this article does not have an abstract, we have provided the first 150 words of the full text PDF and any section headings.

In the past the quantitative determination of histamine in the skin has depended solely upon the pharmacological action of histamine on smooth muscle. The most commonly used pharmacological methods have been to determine the effect on the arterial blood pressure of the cat,¹ or more recently on contraction of gut tissue in vitro. Preliminary acid hydrolysis of the extract has been done by some investigators^* and omitted by others.

Chemical methods for the detection and quantitative determination of histamine have been devised. These all involve preliminary purification and color reactions with various substances. A chemical method, if adequately specific and sensitive, would have definite advantages in ease of performance and avoidance of the problems of a pharmacological method.

The method used in this experiment was an adaptation of that described by Lowry, Graham, and associates¹¹ which has been found to be sensitive and specific when . . . [Full Text PDF of this Article]

Author Affiliations
Cleveland

From the Department of Medicine, Division of Dermatology and Syphilology, Western Reserve University.

Footnotes
Submitted for publication May 27, 1955.

Aided by grant from Research and Development Division, Department of the Army, Contract #DA-49-007-MD-573.

Read before the 75th Annual Meeting of the American Dermatological Association. Inc., Belleair, Fla., April 19, 1955.

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