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Cholinesterases in Human Skin
R. K. WINKELMANN, M.D.;
ROBERT W. SCHMIT
AMA Arch Derm. 1959;80(5):543-548.
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It is now generally accepted that many esterases exist in human skin. Lipases,  -esterases, "Tween" esterases, and specific and nonspecific cholinesterases have been described. Many of the methods are difficult to control and have given results of questionable chemical value. Many authors using these techniques have stated that the histochemical results were hard to evaluate. We wish to report our study of human skin with the Gomori technique, a simple method giving results that are histologically satisfying if not histochemically definite.
Method
The Gomori technique for cholinesterases was used.1 Human skin was excised and placed immediately in cold 10% formalin in 0.9% saline. This was kept at 5 C for three to four hours. Simple formalin solution, acetate-formalin solution, and solutions of 15% sucrose and of 1% ammonium hydroxide in 10% formalin gave much poorer results. The stock solution was the same as that mentioned
. . . [Full Text PDF of this Article]
Author Affiliations
Rochester, Minn.
Footnotes
Submitted for publication March 7, 1959.
This work is supported in part by funds from Public Health Service Grant No. B-1755(C1).
Section of Dermatology (Dr. Winkelmann), Section of Pathologic Anatomy (Mr. Schmit), Mayo Clinic and Mayo Foundation. The Mayo Foundation, Rochester, Minn., is a part of the Graduate School of the University of Minnesota.
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