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HUGO ZAHND, Ph.D.; MARTIN CITRON, M.A.

AMA Arch Derm. 1960;81(6):936-939.

	Since this article does not have an abstract, we have provided the first 150 words of the full text PDF and any section headings.

Introduction

Since the amino acid composition of hydrolysates of psoriatic scales might give some clues as to the pathology of the disease, it was decided to subject the hydrolysates to chromatographic analysis.

The excellent method of column chromatography based on the use of ion exchange resins as developed by S. Moore and W. H. Stein¹ was used in this work. The high degree of percision of the method, as well as the relatively short time required for a complete determination, recommend this procedure.

Method

Preparation of the hydrolysate: The psoriatic scales were washed successively with ether and acetone in order to remove any lipid material which might be present. The washed and air-dried scales were kept in a vacuum dessicator over anhydrous calcium chloride.

The acid hydrolysate was prepared by refluxing the tissue in 15 times its weight of 6 N HCl for a period of from 15 to . . . [Full Text PDF of this Article]

Author Affiliations
Brooklyn, N.Y.

Department of Chemistry, Brooklyn College.

Footnotes
Submitted for publication Aug. 5, 1959.

A preliminary report of this work was presented at the Fourth International Congress of Biochemistry, Vienna, Sept., 1958.

The data in this paper are taken from a thesis submitted by Martin Citron in partial fulfilment of the requirements for the degree of Master of Arts in the Faculty of Liberal Arts and Sciences, Brooklyn College.

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