This Article  • References  • Full text PDF  • Send to a friend  • Save in My Folder  • Save to citation manager  • Permissions  Citing Articles  • Citing articles on HighWire  • Contact me when this article is cited  Related Content  • Similar articles in this journal  Social Bookmarking   What's this?

KARL STEINER, M.D.

Arch Dermatol. 1960;82(3):352-361.

	Since this article does not have an abstract, we have provided the first 150 words of the full text PDF and any section headings.

In two previous studies (Steiner^14,15) it was noticed that in parakeratoses and hyperkeratoses the stratum corneum seems to contain ribonucleic acid (RNA). This observation was made on sections pretreated with ribonuclease (RNase) before staining with methylgreen-pyronin. It was felt, however, that the quantitative differences between RNase-treated and untreated sections were not significant enough to permit a definite conclusion as to the presence of RNA in the stratum corneum. In order to unequivocally demonstrate RNA it appeared desirable to employ a different histochemical method.

In studying hair keratin Jarrett¹¹ showed that RNase abolished the brilliant fluoresscence of thioflavine-stained inner root sheaths. Since such a qualitative change of fluorescence reveals the presence of RNA with certainty, it was decided to apply the fluorescence method to epidermal tissues. In the following, the results of a fluorescence study in regard to the normal and pathologic epidermis and stratum corneum are being described. . . . [Full Text PDF of this Article]

Author Affiliations
Brooklyn

From the Medical Service, Section of Dermatology, Veterans Administration Hospital, and the Department of Medicine, Division of Dermatology and Syphilology, State University of New York, College of Medicine at New York City.

Footnotes
Submitted for publication Jan. 14, 1960.

This study was supported by a grant from the National Institute of Health, U.S.P.H.S. Grant No. RG 4961.

CiteULike    Connotea    Del.icio.us    Digg    Reddit    Technorati    Twitter     What's this?