This Article  • References  • Full text PDF  • Send to a friend  • Save in My Folder  • Save to citation manager  • Permissions  Citing Articles  • Citing articles on HighWire  • Citing articles on Web of Science (8)  • Contact me when this article is cited  Related Content  • Similar articles in this journal  Social Bookmarking   What's this?

An Application of Ultramicrotitration and Volume Measurement Methods to a Quantitative Study of the Exopeptidases in Skin Sections

J. HASEGAWA, M.D.

Arch Dermatol. 1960;82(4):595-604.

	Since this article does not have an abstract, we have provided the first 150 words of the full text PDF and any section headings.

Introduction

In clinical medicine the most readily available biopsy material, the blood, has become the major source for quantitative chemical determinations in the diagnosis and treatment of disease. In dermatology the skin, another accessible tissue for biopsy, has not yet been utilized for clinical chemical measurements. The development of the skin biopsy as a source for clinical chemical study awaits the elimination of two obstacles. The first is the small size of the biopsy tissue ordinarily available. Unlike the blood, chemical determinations of the skin must be measured in specimens considerably smaller than 0.1 cc. in volume. In order to overcome this difficulty it is necessary to employ microchemical methods. Numerous micromethods have been devised, and, in fact, have been applied to chemical determinations of the blood in hospital laboratories.¹ The second obstacle to the use of skin biopsy specimens is the heterogeneity of the skin. The human skin, . . . [Full Text PDF of this Article]

Author Affiliations
Chicago

From the Northwestern University Medical Center, Department of Dermatology (Herbert Rattner, M.D., Chairman).

Footnotes
Submitted for publication March 18, 1960.

Awarded fourth prize in the 1959 Essay Competition of the American Dermatological Association.

This investigation was supported in part by a research grant RG-6797 from the Division of General Medical Sciences, U.S. Public Health Service.

CiteULike    Connotea    Del.icio.us    Digg    Reddit    Technorati    Twitter     What's this?