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HERMANN PINKUS, M.D.; ROSIE HUNTER

Arch Dermatol. 1960;82(5):699-700.

	Since this article does not have an abstract, we have provided the first 150 words of the full text PDF and any section headings.

The normal and pathological skin presents a variety of tissue elements that are not demonstrated or not easily differentiated in sections stained with the routine hematoxylin and eosin (H and E) combination. In 1944, a modification of Unna-Taenzer's method for elastic fibers and cellular elements was described,¹ which combined relative ease and great reliability with differential staining of more skin constituents than can be visualized with the original method. In recent years, a new type of synthetic orcein has become available and has made further simplification of the technique possible.

Tissues may be fixed in a dilute formalin, 70% or absolute alcohol, or formol-alcohol. Fixatives containing chromates, mercury, or picric acid should be avoided. Paraffin sections may be from 5_µ to 15_µ thick. Sections 10_µ in thickness often are preferable to thinner ones, as they give a better threedimensional picture.

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Author Affiliations
Detroit

From the Department of Dermatology, Wayne State University College of Medicine.

Footnotes
Submitted for publication May 10, 1960.

Supported in part by research grants from the National Institutes of Health, U.S. Public Health Service, and by a research contract from the U.S. Army, Office of the Surgeon General.

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