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ERNEST W. CHICK, M.D.

Arch Dermatol. 1961;83(2):305-309.

	Since this article does not have an abstract, we have provided the first 150 words of the full text PDF and any section headings.

Introduction

Acridine orange has been used for some time as a fluorescent stain for nucleoproteins and for tissue mucopolysaccharides.⁶ It has also found use as a vital stain for blood parasites.⁸

By accident it was found that the technique of Hicks and Matthaei for the fluorescent staining of mucin⁴ would also stain fungi in tissue sections which would then fluoresce under blue-violet light.⁷ This finding led to a study of further applications of this method to demonstrate structural details of the fungi themselves and as diagnostic aids in clinical evaluations.

Materials and Methods

Microscope and Filters.—

These studies were done using a Reichert Zetopan microscope with fluorescent attachments. Results were checked periodically with an ordinary binocular microscope and lamp equipped with filters for fluorescent microscopy⁷ to insure adaptability to this apparatus. In the latter apparatus a Leitz or Zeiss bluelight fluorescence filter (BG 12, 4 mm. . . . [Full Text PDF of this Article]

Author Affiliations
DURHAM, N.C.

Associate in Pathology, Duke University School of Medicine and Clinical Investigator, Veterans Administration Hospital (Dr. Chick).

From the Department of Pathology, Duke University School of Medicine, and the Laboratory Service, Veterans Administration Hospital, Durham, N.C.

Footnotes
Submitted for publication Sept. 12, 1960.

Presented before the section on Pathology, Medical Society of the State of North Carolina, Raleigh, N.C., May 10, 1960.

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