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Determination by Use of Fluorescent Antibody Technique

JOAN RASKIN, M.D.

Arch Dermatol. 1961;83(3):459-465.

	Since this article does not have an abstract, we have provided the first 150 words of the full text PDF and any section headings.

The exact site of the antigen-antibody reaction in allergic contact dermatitis is unknown. Early investigators were proponents of the humoral theory, believing that the site of the antigen-antibody reaction is in the blood stream. Supporters of the currently more popular cellular theory offer evidence to indicate that the site is in or on the tissue cells.¹⁶ In 1942, Coons and co-workers first demonstrated the value of using a fluorescent antibody to identify antigens in tissue.¹ In the past decade there have been many refinements in the procedure,³ and the technique has been used successfully in the identification of micro-organisms^5,6,10,11 and in the immunohistochemical investigation of connective tissue diseases and many other pathologic states.^14,17 This paper reports the successful results of application of the technique to localize the site of the antigenantibody reaction in human subjects in experimentally produced Rhus dermatitis.

Materials and Methods

A. . . . [Full Text PDF of this Article]

Author Affiliations
BALTIMORE

From the Division of Dermatology, University of Minnesota, Dr. Francis W. Lynch, Director.

Formerly Fellow, Division of Dermatology, University of Minnesota. Presently Associate in Dermatology, University of Maryland, School of Medicine.

The staff and patients of the Hastings State Hospital cooperated in the study.

Dr. Rudolph Blazejovsky cut the sections.

Footnotes
Submitted for publication July 5, 1960.

Supported by grant from Mr. T. H. Rowell and Rowell Laboratories, Inc., Baudette, Minn., and Institutional Grant of the University of Minnesota provided by the Minnesota Division of the American Cancer Society, Inc.

Goat antihuman conjugate and electrophoretic study provided by the Baltimore Biological Laboratory, Inc., Baltimore, Md.

Fluorescein isothiocyanate courtesy of Dr. Morris Goldman, Communicable Disease Center, Atlanta, Ga.

Rhus oleoresin courtesy of Hugh Graham, Inc., Dallas, Texas.

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