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Fluorescent Antibody Studies in Chronic Dermatitis
LOUIS J. WISE, JR., M.D.;
JAY M. SHAMES;
VINCENT J. DERBES, M.D.;
FRED M. HUNTER, M.D.
Arch Dermatol. 1961;84(1):37-39.
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| Since this article does not have an abstract, we have provided the first 150 words of the full text PDF and any section headings. |
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The fluorescent antibody technique originated by Dr. Albert H. Coons1 provides an excellent method for localization of antigen-antibody reactions in tissue sections. Globulin molecules are conjugated with a dye which renders them fluorescent when viewed through a special microscope. The antibody, thus labeled, is employed as a histochemical stain for the demonstration of antigen.2
The method is applicable either as an indirect method utilizing tagged antiglobulin antiserum to demonstrate prefixed antibody or as a direct method in which specific antiserum is tagged and reacted directly with the antigen. With either method, one is obviously dealing with a spectrum of antigen-antibody systems unless specific absorptions are performed, and the results must be interpreted accordingly.
In the following study the direct method of Coons was employed in selected cases of chronic dermatitis. Fourteen patients were tested using labeled antiserum and autologous skin sections.
Method
A detailed account of the procedure
. . . [Full Text PDF of this Article]
Author Affiliations
NEW ORLEANS
Department of Medicine, Division of Dermatology and Division of Gastroenterology, Tulane University School of Medicine and Charity Hospital, senior student, Tulane University School of Medicine (Mr. Shames).
Footnotes
Submitted for publication March 8, 1961.
This work was supported by Research Grant A-2838 from the National Institute of Health, U. S. Public Health Service.
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