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Thomas K. Burnham, MB, BS; Thomas R. Neblett, PhD; Gerald Fine, MD
Henry Ford Hospital Detroit 48202

Arch Dermatol. 1969;99(3):364-365.

	Since this article does not have an abstract, we have provided the first 150 words of the full text PDF and any section headings.

To the Editor.—

We wish to comment on Drs. Beck and Rowell's¹ reply to our suggestion² that failure to differentiate true speckles from "speckle-like threads" might be the reason why speckled fluorescence was seen equally frequently in SLE and scleroderma by Drs. Beck and Rowell³ whereas we found it almost exclusively confined to scleroderma^4,5 and Raynaud's disease.^2,6

1. 1. Although we investigated various parameters such as length of storage of tumors before freezing and desirability of autopsy versus surgically obtained human tumors in our initial reports of the immunofluorescent tumor imprint technique4,7 we use only surgically obtained specimens which are frozen and/or imprinted within one to two hours of excision. We reported4 that autopsy material was less desirable than surgical specimens and, of course, agree with Drs. Beck and Rowell that autolyzed tissue should not be used.
   
2. 2. The fact that different types of tumors are
   

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